Abstract Details Title A Computational Model of the Potential MicroRNAs in Ischemic Stroke and Relation to Endothelial Dysfunction by SARS-CoV-2 with Theoretical Nuclear Physics Methods Topic Cerebrovascular Disease and Interventional Neurology Presentation(s) Cerebrovascular Disease and Interventional Neurology Posters (7:00 AM-5:00 PM) Poster/Presentation Number 024 Objective To elaborate a computational model of the potential microRNAs in ischemic stroke and relation to endothelial dysfunction by SARS-CoV-2 with theoretical nuclear physics methods. Background Ischaemic stroke has increasingly been reported as a complication of COVID-19 infection, particularly in more severe cases, being associated with the affinity of the SARS-CoV-2 for ACE2 receptors, which are expressed in endothelial and arterial smooth muscle cells in the brain. Design/Methods The computational analysis was elaborated based on (1) the intra-neuronal space, the intra-glial space and the extracellular space; (2) mathematical model of ion movements in grey matter during a stroke; (3) the role of the blood-brain barrier in stroke; (4) modelling of pH dynamics in brain cells after stroke; (5) structures, functions and physicochemical properties of potential microRNAs in ischemic stroke; (6) structural biology of hetero-oligomeric complex of spike glycoprotein/human ACE2 and structural and functional properties of SARS-CoV-2 spike protein; (7) inflammatory cytokines in stroke and SARS-CoV-2 infection. Gene Expression Omnibus (GEO) database, the database of miRTarBase, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment were applied to interpret the function of the miRNA targets. Computational simulations and analyzes were elaborated with the use of software: ACD/ChemSketch, Swiss-PdbViewer, ABCpred, BepiPred-2.0, FunRich, Cytoscape, BiNGO, GROMOSA7, GROMACS 5.1, MolAr, AutoDock Vina, Visual Molecular Dynamics (VMD), Cell Illustrator, GENESIS, NEURON, NeuronStudio and ChemDraw. Results The computational model of this work suggests that miR-145 and miR-122 may represent potential biomarkers in ischemic stroke as a complication of COVID-19 infection by being involved in the process of postischemic neuronal damage and thrombosis, respectively. This research also suggests that miR-99b, miR-542-3p and miR-455-5p play role in the pathophysiological mechanisms of ischemic stroke due to SARS-CoV-2 infection. Conclusions Understanding the pathophysiological mechanisms and relations between the ischaemic stroke and SARS-CoV-2 infection should help developing new therapeutic tools to treat this clinical condition. Authors/Disclosures João Marcos Brandet PRESENTER Mr. Brandet has nothing to disclose.