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Abstract Details

Muscle Satellite Cell Ablation Leads to Disorganization of Muscle Microvasculature
General Neurology
P13 - Poster Session 13 (5:30 PM-6:30 PM)
6-009
We hypothesize that aberrant microvascular patterning due to muscle satellite cell (MuSC) depletion exacerbates muscle degeneration. 
Skeletal muscle is a highly ordered yet complex tissue containing several cell types that interact with each other in order to maintain structure and homeostasis. Of the various types of cells, MuSCs, the muscle tissue resident stem cell, are the most important for muscle growth, repair and regeneration. Decline of the MuSC pool has been implicated in several diseases, such as Duchenne Muscular Dystrophy (DMD), where the muscle’s regenerative demands outpace the repletion of the MuSC pool. 
Proper examination of this process requires one to look at the 3-dimensional orientation of the cellular components, which can be accomplished through tissue clearing. Recently, we have optimized a tissue clearing protocol for skeletal muscle and utilized fluorescent reporters for endothelial cells (Flk1-GFP) and MuSCs (Pax7CreERT2:R26R-Stop-TdTomato) along with tissue clearing to investigate the proximity of MuSCs to blood vessels in 3-dimensions.
We have recently shown that MuSCs are not only vital for muscle regeneration, but also necessary for the maintenance of the muscle microvasculature. We found that ablation of MuSCs via Diphtheria Toxin fragment-A (DTA) in Pax7CreERT2:R26R-Stop-DTA mice leads to disorganization of the muscle vasculature, quantified by measuring vessel density and mean tortuosity index versus control. 
Our findings suggest that MuSCs play a role in the patterning of the microvasculature of muscle. Further studies into the impact of the vessel disorganization on muscle function will further clarify the effects of MuSC depletion in muscle degenerative diseases.
Authors/Disclosures
Kyutae Kim
PRESENTER 		No disclosure on file
No disclosure on file
No disclosure on file
Mayank Verma, MD, PhD (UTSW) No disclosure on file
No disclosure on file