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Abstract Details

Serum Neurofilament Light Chain Concentrations Withstand Delayed Freezing up to 24 Hours – a Pre-analytical Study.
General Neurology
P13 - Poster Session 13 (5:30 PM-6:30 PM)
6-010
To determine the pre-analytical stability of serum neurofilament light chain (sNfL) concentrations, we investigated the influence of delayed freezing on measured sNfL levels.

Neurofilaments have aroused considerable attention in biomarker research in a variety of neurological disorders. With an increasing rate of sNfL research, there is an immanent need to acquire knowledge about the influence of pre-analytical blood processing with respect to varying pre-freezing intervals.

We recruited 30 patients from our neurological outpatient department who consented to a single blood sampling for this study. From each patient, we collected three 9-milliliter serum tubes which underwent different methods of processing: (i) the first tube was centrifuged, aliquoted and frozen at -80°C within thirty minutes. The second and third tubes were stored in a conventional refrigerator (+6°C) for (ii) three and (iii) 24 hours, respectively; both before being processed further. sNfL concentration was measured using Simoa-technology. Reproducibility of sNfL results after all three pre-freezing intervals was assessed by calculating mean differences and 95% confidence intervals, intraindividual coefficients of variation and intraclass correlation coefficients (ICC; with an ICC of 1 representing maximum reproducibility).
Mean differences in sNfL concentrations compared to immediate freezing were 0.3 pg/mL (-1,1–1.7; p=0.658) after three hours of delayed freezing and 0.3 pg/mL (-0.8–1.3; p=0.632) after 24 hours. For both pre-freezing intervals, intraindividual variation was minimal (3h: 0.1%; 95% CI 0.0–0.1 and 24h: 0.1%; 95% CI 0.0–0.1) and reproducibility nearly perfect (3h: ICC=0.98; 95% CI 0.95–0.99 and 24h: ICC=0.98;  95% CI 0.95–0.99).
Serum tubes can be stored up to 24 hours in a refrigerator (+6°C) before being processed to -80°C and still yield stable results for sNfL concentrations. Future methodical study considerations shall appreciate this observation.
Authors/Disclosures
Patrick Altmann (Univ.-Klinik für Neurologie)
PRESENTER 		The institution of Mr. Altmann has received research support from Quanterix. The institution of Mr. Altmann has received research support from Biogen, Merck, Roche, Sanofi, Teva,. Mr. Altmann has received intellectual property interests from a discovery or technology relating to health care.
Fritz Leutmezer Fritz Leutmezer has received personal compensation in the range of $5,000-$9,999 for serving as a Consultant for Almirall, Biogen, Celgene, Johnson&Johnson, Novartis, Merck, Roche, Sanofi, Teva-Ratiopharm. Fritz Leutmezer has received personal compensation in the range of $5,000-$9,999 for serving on a Scientific Advisory or Data Safety Monitoring board for Almirall, Biogen, Celgene, Johnson&Johnson, Novartis, Merck, Roche, Sanofi, Teva-Ratiopharm. Fritz Leutmezer has received personal compensation in the range of $10,000-$49,999 for serving on a Speakers Bureau for Almirall, Biogen, Celgene, Johnson&Johnson, Novartis, Merck, Roche, Sanofi, Teva-Ratiopharm. The institution of Fritz Leutmezer has received research support from Biogen, Merck, Novartis, Roche.
No disclosure on file
No disclosure on file
No disclosure on file
Henrik Zetterberg (Sahlgrenska University Hospital/Molndal) Henrik Zetterberg has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Abbvie, Acumen, Alector, Alzinova, ALZpath, Amylyx, Annexon, Apellis, Artery Therapeutics, AZTherapies, Cognito Therapeutics, CogRx, Denali, Eisai, LabCorp, Merry Life, Nervgen, Novo Nordisk, Optoceutics, Passage Bio, Pinteon Therapeutics, Prothena, Quanterix, Red Abbey Labs, reMYND, Roche, Samumed, Siemens Healthineers, Triplet Therapeutics, and Wave. Henrik Zetterberg has received personal compensation in the range of $500-$4,999 for serving on a Speakers Bureau for Alzecure, BioArctic, Biogen, Cellectricon, Fujirebio, Lilly, Novo Nordisk, Roche, and WebMD. Henrik Zetterberg has received personal compensation in the range of $500-$4,999 for serving as an Editor, Associate Editor, or Editorial Advisory Board Member for Wiley and Elsevier. Henrik Zetterberg has stock in Brain Biomarker Solutions.
Paulus S. Rommer (AKH Wien / Univ. Klinik Fuer Neurologie) Dr. Rommer has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Alexion. Dr. Rommer has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Roche. Dr. Rommer has received personal compensation in the range of $500-$4,999 for serving as a Consultant for biogen. The institution of Dr. Rommer has received personal compensation in the range of $500-$4,999 for serving as a Consultant for celgene. The institution of Dr. Rommer has received personal compensation in the range of $500-$4,999 for serving as a Consultant for merck. Dr. Rommer has received personal compensation in the range of $0-$499 for serving as a Consultant for sanofi. Dr. Rommer has received personal compensation in the range of $500-$4,999 for serving on a Scientific Advisory or Data Safety Monitoring board for sandoz. Dr. Rommer has received personal compensation in the range of $500-$4,999 for serving on a Speakers Bureau for Abbvie. Dr. Rommer has received personal compensation in the range of $500-$4,999 for serving on a Speakers Bureau for almirall. Dr. Rommer has received personal compensation in the range of $500-$4,999 for serving on a Speakers Bureau for biogen. Dr. Rommer has received personal compensation in the range of $500-$4,999 for serving on a Speakers Bureau for Novartis. Dr. Rommer has received personal compensation in the range of $500-$4,999 for serving on a Speakers Bureau for Merck. The institution of Dr. Rommer has received research support from merck. The institution of Dr. Rommer has received research support from roche. The institution of Dr. Rommer has received research support from amicus. The institution of Dr. Rommer has received research support from biogen.
Thomas A. Berger, MD (Dept. of Neurology, Medical University of Vienna) Prof. Berger has received personal compensation in the range of $10,000-$49,999 for serving as a speaker at scientific meetings and participant of local and international advisory boards with various companies producing and markerting treatments for multiple sclerosis (Almirall, Biogen, Biologix, Bionorica, Celgene-BMS, Merck, Novartis, Roche, Sanofi-Genzyme, TG Therapeutics, UCB).
Gabriel Bsteh, MD (Medical University Vienna, Department of Neurology) Dr. Bsteh has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Biogen. Dr. Bsteh has received personal compensation in the range of $500-$4,999 for serving as a Consultant for BMS. Dr. Bsteh has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Novartis. Dr. Bsteh has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Roche. Dr. Bsteh has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Teva. Dr. Bsteh has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Sanofi.